UJI ANTIBAKTERI EKSTRAK BATANG GAHARU (Aquilaria malaccensis) BERBASIS KOLIN KLORIDA:ASAM SITRAT TERHADAP Bacillus cereus dan Shigella dysenteriae
Abstrak
Infeksi yang disebabkan oleh Bacillus cereus dan Shigella dysenteriae merupakan masalah kesehatan serius karena dapat menyebabkan gangguan saluran pencernaan serta meningkatkan risiko resistensi antibiotik. Pendekatan yang dilakukan untuk mengatasi masalah ini adalah dengan mengembangkan agen antibakteri baru berbasis bahan alam, seperti tanaman gaharu (Aquilaria malaccensis). Penelitian ini bertujuan untuk mengevaluasi aktivitas antibakteri ekstrak batang gaharu yang diperoleh menggunakan Natural Deep Eutectic Solvent (NADES) berbahan dasar kolin klorida dan asam sitrat melalui metode Ultrasound Assisted Extraction (UAE). Uji antibakteri dilakukan dengan metode difusi cakram menggunakan berbagai rasio NADES (1:1, 2:1, 1:2, 1:3, 1:4, dan 4:1 g/g), dengan kloramfenikol sebagai kontrol positif dan DMSO 10% sebagai kontrol negatif. Hasil menunjukkan bahwa rasio NADES 1:4 g/g menghasilkan zona hambat terbesar terhadap pertumbuhan Bacillus cereus dan Shigella dysenteriae. Selain itu, uji Konsentrasi Hambat Minimum (KHM) menunjukkan bahwa ekstrak dengan rasio NADES 1:4 mampu menghambat pertumbuhan kedua bakteri pada konsentrasi 30%. Temuan ini mengindikasikan bahwa ekstrak batang gaharu dengan pelarut NADES berpotensi sebagai agen antibakteri yang efektif dan ramah lingkungan.
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