SKRINING FITOKIMIA DAN UJI ANTIOKSIDAN TERHADAP EKSTRAK BUNGA Limnocharis flava L DENGAN METODE DPPH

Ermi Abriyani; Lia Fikayuniar; Mia Anisa Silvi; Arie Wichandar

doi:10.36805/jbf.v2i2.392

Ermi Abriyani Fakultas Farmasi, Universitas Buana Perjuangan Karawang, Jawa Barat, Indonesia
Lia Fikayuniar Fakultas Farmasi, Universitas Buana Perjuangan Karawang, Jawa Barat, Indonesia
Mia Anisa Silvi Fakultas Farmasi, Universitas Buana Perjuangan Karawang, Jawa Barat, Indonesia
Arie Wichandar Fakultas Farmasi, Universitas Buana Perjuangan Karawang, Jawa Barat, Indonesia

DOI: https://doi.org/10.36805/jbf.v2i2.392

Keywords: Limnocharis flava (L.), screening phytochemical, antioxidant, Metode DPPH

Abstract

Genjer (Limnocharis flava L.) is of plants that bloom throughout the year. The part that can be used of Limnocharis flava L. is leaves, but more people used stems and flowers of Limnocharis flava L. for consumption. The present study has aim to determine the phytochemical screening and antioxidants activity assay of Limnocharis flava L. bloom extracts. Method of the research are extraction by maceration, screening phytochemical, DPPH method which is by measuring the capture of synthetic radicals in polar organic solvents at room temperature. The results of phytochemical screening of Limnocharis flava L. bloom are flavonoid, saponin, tannin, polyphenols, quinone. Antioxidant activity assay of Limnocharis flava L. bloom from three extracts have resulted IC₅₀ value from methanol extract 61.4224 ppm, ethyl acetate extract 160.4368 ppm and n-hexane 433,2932 ppm. Methanol extract has a stronger antioxidant compared to ethyl acetate and n-hexane extracts by DPPH method.

References

Abriyani, E., & Fikayuniar, L. (2020). Screening Phytochemical , Antioxidant Activity and Vitamin C Assay from Bungo perak-perak ( Begonia versicolar Irmsch ) leaves. 10(3), 1–5.
Bergh MH. 1994. Limnocharis flava (L) Buchenau. Di dalam: Siemonsma JS dan Piluek K, editor. Plant Resources of South-East Asia. Bogor: Prosea. hlm 192-194
Harborne. (1987). Metode Fitokimia, Penentuan Modern Menganalisa Tumbuhan, Terbitan ke-2. Terjemahan Padmawinata, K. dan Soediro, I. Bandung: Penerbit ITB.
Keng-fei Ooh, Hean-Chooi Ong, Fai-Chu Wong dan Tsun-Thai Chai, (2015), hplc profiling of phenolic acids and flavonoids and evaluation of anti-lipoxygenase and antioxidant activities of aquatic vegetable limnocharis flava,

Kusumaningsih, Triana., et.al. 2015. Pengurangan Kadar Tanin Pada Ekstrak Stevia Rebaudiana Dengan Menggunakan Karbon Aktif. Alchemy Jurnal Penelitian Kimia Vol. 11

Molyneux, P., 2004, The Use of The Stable Free Radical Diphenylpicryl-hydrazyl (DPPH) for Estimating Antioxidant Activity, Songklanakarin J. Sci. Technol. , 26(2), 211-21

Prakash, A., 2001, Antioxidant Activity, Medallion Laboratories Analytical Progress, vol. 19, No.2.
Sediaoetama,A.D. 1993. Ilmu Gizi Jilid II. Dian Rakyat. Jakarta.
Shivaprasad, H.N., Mohan, S., Kharya, M.D., 2005. In-vitro model for antioxidant activity evaluation. a review.
Sirwutubun, M., M. M. Ludong, D. Rawung. 2016. Pengaruh Konsentrasi Etanol terhadap Karakteristik Ekstrak Pewarna Alami Buah Merah (Pandanus conoideus Lamk) dan Aplikasinya pada Produk Pangan. Fakultas Pertanian UNSRAT.
Tiwari, P., Kumar, B., Kaur, M., Kaur G. & Kaur H., 2011, Phytochemical Screening And Extraction: A Review, International Pharmaceutica Sciencia, 1 (1), 98-106.