UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL PADA BUAH BIT (Beta vulgaris L.) MENGGUNAKAN METODE DPPH (2,2-DIPHENYL-1-PICRYLHYDRAZIL)
Abstract
Oxidative stress due to the accumulation of free radicals plays a role in the development of various degenerative diseases, so that natural antioxidant sources that can be scientifically evaluated are needed. Beetroot (Beta vulgaris L.) is known to contain bioactive compounds such as betalains, flavonoids, and polyphenols that have the potential as antioxidants. This study aims to evaluate the antioxidant activity of beetroot (Beta vulgaris L.) ethanol extract in vitro using the DPPH method. Extraction was carried out by maceration method using 96% ethanol. The antioxidant activity test was carried out using DPPH solution with absorbance measurement at a wavelength of 517 nm. The extract was tested at a concentration range of 10–100 µg/mL with three replications, while quercetin was used as a positive control. Antioxidant activity was expressed as the percentage of DPPH radical inhibition and the IC₅₀ value calculated using linear regression analysis. The results showed that the beetroot ethanol extract had an IC₅₀ value of 3.35 µg/mL, while quercetin had an IC₅₀ value of 2.76 µg/mL. The yield of the extract obtained was 20.29%. These results indicate that the beetroot ethanol extract has DPPH radical scavenging activity in the in vitro test system used.
References
Aisyah, P. N., Rahma, A., & Sutrisno Adi Prayitno. (2025). ANALISIS KADAR KUERSETIN DAN MUTU SENSORI PADA FORMULASI MINUMAN SEDUHAN BERBAHAN DASAR DAUN KELOR DAN BUAH BELIMBING WULUH. 2(2), 144–155.
Ginting, A. N. B., Kaban, V. E., Banga, R. I., & Daimah W. S. Harahap. (2025). Formulasi dan Uji Aktivitas Antibakteri Gel Minyak Atsiri Rimpang Lengkuas Merah (Alpinia purpurata K. Schum) terhadap Propionibacterium acnes. INSOLOGI: Jurnal Sains dan Teknologi, 4(1), 75–88. https://doi.org/10.55123/insologi.v4i1.4844
Lanes, C. A., Yudistira, A., & Meilani Jayanti. (2023). ANTIOXIDANT ACTIVITY TEST OF Lamellodysidea herbacea SPONGES ETHANOL EXTRACT OBATAINED FROM MINAHASA REGENCY SOUTH BEACH. 12, 367–372.
miranti. (2023). miranti. 2023. “Pend_Klt.” 1(2):8–13. 1(2), 8–13.
Sepriyani, H., Devitria, R., Surya, A., & Sari, S. (2020). AKTIVITAS ANTIOKSIDAN EKSTRAK METANOL DAUN PEPAYA ( Carica papaya L ) DENGAN METODE. Jurnal Penelitian Farmasi Indonesia, 9(1), 2018–2021.
Sihotang, N., Bangar, R. I., Kaban, vera E., Sembiring, N. B., Sanaun, D. W., & Harahap. (2025). Antioxidant Activity Test of The Ethyl Acetate Fraction of Tetanus Leaf (Leea aequata L.) Using The DPPH Method. PCJN: Pharmaceutical and Clinical Journal of Nusantara, 3(01), 21–25. https://nusantarascientificjournal.com/index.php/pcjn/article/view/88
Sylvia Winata, Rena Meutia, Astriani Natalia, & Asyrun Alkhairi Lubis. (2025). Avocado Seed Ethanol Extract’s Ability to Reduce Hyperuricemia in Mice. Jurnal Indah Sains dan Klinis, 6(1), 01–08. https://doi.org/10.52622/jisk.v6i1.01
Vifta, R. L., Marini, Y., Puspitasari, A. D., Badriyah, L., & Sulastri, S. (2025). Analisis Flavonoid Total Ekstrak Buah Bit (Beta vulgaris L.) Berdasarkan Metode dan Lama Ekstraksi Secara Spektrofotometri. Generics: Journal of Research in Pharmacy, 5(1), 86–98. https://doi.org/10.14710/genres.v5i1.25639
Yuli Kurniasari, Kharismatul Khasanah, Vera Yunita, Labibah Alawiyah, & Puji Wijayanti. (2023). Aktivitas Antioksidan Ekstrak Serbuk Bekatul Menggunakan Metode Dpph, Abts, Dan Frap. CERATA Jurnal Ilmu Farmasi, 13(2), 82–90. https://doi.org/10.61902/cerata.v13i2.612
Zuniarto, A. A., Pandanwangi, S., Nuari, R. A., & Listya, D. G. (2015). Antioxidant Activity Of Nanoemulsion Of Beet Fruit Extract Using The Dpph Method. 19(1).
