UJI AKTIVITAS ANALGESIK FRAKSI DAUN SENGGANI  (Clidemia hirta [L] D.Don) PADA MENCIT PUTIH GALUR SWISS WEBSTER

Taufik Septiyan Hidayat; Dadan Rohdiana; Syumillah Saepudin; Dini Febrianty; Yunita Al Azzahra

doi:10.36805/jbf.v4i1.935

Taufik Septiyan Hidayat Program Studi Farmasi, Fakultas MIPA Universitas Al Ghifari, Bandung, Jawa Barat Indonesia
Dadan Rohdiana Program Studi Farmasi, Fakultas MIPA Universitas Al Ghifari, Bandung, Jawa Barat Indonesia
Syumillah Saepudin Program Studi Farmasi, Fakultas MIPA Universitas Al Ghifari, Bandung, Jawa Barat Indonesia
Dini Febrianty Program Studi Farmasi, Fakultas MIPA Universitas Al Ghifari, Bandung, Jawa Barat Indonesia
Yunita Al Azzahra Akademi Farmasi Bumi Siliwangi, Bandung, Jawa Barat, Indonesia

DOI: https://doi.org/10.36805/jbf.v4i1.935

Keywords: Acetic acid, Analgesic, Dexamethasone, Senggani fleaves

Abstract

Pain is a sensation that can interfere with activities and reduce the quality of life of the sufferer if left untreated and left prolonged. Senggani is one of the plants that contains flavonoid compounds and steroids that have analgesic activity. This study aimed to test the analgesic activity of senggani leaf fraction (Clidemia hirta [L] D.Don) in white mice swiss webster strain induced acetic acid and determine the fraction that most effectively produces analgesic activity. The type of research conducted is true experimental design based on Complete Randomized Design (RAL). The test animals used were 25 heads divided into 5 groups with 5 replications, namely negative control (CMC 1%), positive control (dexamethasone) and the test group of senggani leaf fraction, namely n-hexane, ethyl acetate, and ethanol fractions. The data obtained were analyzed using an alternative test from One Way ANOVA namely Kruskal Wallis with a follow-up test post hoc Mann Whitney test. The results showed that the fraction of senggani leaves in all types of solvents had analgesic activity compared to negative controls (CMC 1%) with a significant difference (p0.05). From these results it is concluded that the senggani leaf fraction can be used as an analgesic.

References

Ali, A.H.A., Rinidar, Armansyah, T., Rosmaidar, Harris, A., dan Dasrul. (2016). Potensi Ekstrak Daun Sernai (Wedelia biflora) Sebagai Antinyeri Pada Mencit (Mus musculus). Jurnal MedikaVeterinaria. 10 : 137-140.

Alkandahri MY, PN Siahaan, Salim E, Fatimah C. AntiInflammatory activity of Cep-cepan leaves (Castanopsis costata (Blume) A.DC). Int J Curr Med Sci. 2018;8(4A):424-429.

Amalia A, Romdon DS, Kusumawati N, Salsabila MA, Afifah ND, Shaomitha R, et al. Antihyperuricemia activities of several active compounds from medicinal plants: A review. Eur J Biomed Pharm Sci, 2024; 11(3): 15-18.

Andini DAP, Utami DSP, Puspawati I, Geralda AY, Apriani IP, Syalomita D, et al. Antidyslipidemic activity of some medicinal plants from Karawang, West Java, Indonesia: A review. Eur J Pharm Med Res, 2024; 11(3): 1-4.

Anwar, Khoerul., Riswandi, Muhammad., Nurlely. (2019). Perbandingan Aktivitas Analgetik Infusa dan Ekstrak Etanol Umbi Akar Tawas Ut (Ampelocissus rubiginosa Lauterb.). Jurnal Pharmascience, Vol. 06 , No.02, Oktober 2019, hal: 40 – 47.

Budiyanti LE, Saputra MYKA, Wulandari SA, Amallia S, Azzahra AJ, Sagala BC, et al. Antihypertensive effects of some medicinal plants in Indonesia: A review. Eur J Pharm Med Res, 2024; 11(3): 5-8.

Dewi, Sisilia Rosmala., dan Salim, Hiany. (2018). Uji Efek Analgetik Infusa Jahe (Zingiber Officinale Roscoe) Terhadap Hewan Uji Mencit Jantan (Mus musculus). Media Farmasi p.issn 0216- 2083 e.issn 2622-0962 Vol. XV No. 2, Oktober 2018.

Halima, Susanna., Ermi, Girsangb., I Nyoman Ehrich Listerc., & Ali, Napiah Nasutiond. (2019). Effectivity of Gel Ethanolic Extract of Senggani Leaves (Melastoma candidum D. Don) in Increasing the Number of Fibroblast Cells and Thickness of Collagen Fibers Against Socket Wound after Tooth Extraction on Male White Rats. American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) ISSN 2313-4410, ISSN 2313-4402.

Keswara, Yane Dila., Handayani., Sri Rejeki. (2019). Uji Aktivitas Analgetik Ekstrak Etanol Daun Inggu (Ruta angustifolia [l.] Pers) Pada Tikus Putih Jantan. Journal Syifa Sciences and Clinical Research Volume 1 Nomor 2, September 2019.

Khan, B. A., Ahmad, S., Khan, M. K., Hosny, K. M., Bukhary, D. M., Iqbal, H., Murshid, S. S., Halwani, A. A., Alissa, M., & Menaa, F. (2022). Fabrication and Characterizations of Pharmaceutical Emulgel Co-Loaded with Naproxen-Eugenol for Improved Analgesic and Anti-Inflammatory Effects. Gels, 8(10), 608.

Lara, AD, Elisma, Sani F. (2021) Uji aktivitas analgesik infusa daun jeruju (Acanthus ilicifolius L.) Pada mencit putih jantan (Mus musculus). Ind J Pharma Science, 3(2): 71-80..

Lee, Y. M., Son, E., Kim, S.-H., & Kim, D.-S. (2022). Anti-Inflammatory and Analgesic Effects of Schisandra chinensis Leaf Extracts and Monosodium Iodoacetate-Induced Osteoarthritis in Rats and Acetic Acid- Induced Writhing in Mice. Nutrients, 14(7), 1356. https://doi.org/10.3390/nu14071356.

Low, Jun Sheng., Kit-Kay, Mak., Mallikarjuna, Rao Pichika., Puvaneswari, Marappan., Shiming Zhang.,Apiwat, Jiampanichkul., Servenna Chua., Kavitha Mohandas., & Madhu Katyayani Balijepalli. (2021). Melastoma Malabathricum L.: A Review Of Its Traditional Uses, Phytochemical Constituents And Bioactivities. Journal of Global Trends in Pharmaceutical Sciences ISSN- 2230-7346 12 (2): 9257– 9274.

Maravirnadita, Ariadna Hisela. (2019). Uji Aktivitas Antioksidan Fraksi N-Heksan, Etil Asetat, Dan Air Dari Buah Belimbing Manis (Averrhoa Carambola) Dengan Metode DPPH. Yogyakarta : Universitas Ahmad Dahlan.

Nosek, K., Leppert, W., Puchała, Ł., & Łoń, K. (2022). Efficacy and Safety of Topical Morphine: A Narrative Review. Pharmaceutics, 14(7), 1499. https://doi.org/10.3390/pharmaceutics14 071499.

Noval dan Ali, R. H. (2014). Uji Aktivitas Antipiretik Infusa Daun Mahkota Dewa

(Phaleria Macrocarpa (Scheff) Boerl.) Pada Tikus Putih Galur Wistar. Banjarmasin : Sekolah Tinggi Ilmu Kesehatan Sari Mulia.

Noviyanty, Yuska., Reni, Febrianti., Hepiyansori. (2022). Ekstraknasi Dan Skrining Ekstrak Ekstrak Etanol Daun Binahong (Anredera Cordifolia (Ten) Steenis) Dengan Menggunakan Metode Kromatografi Lapis Tipis. Jurnal Ilmiah Pharmacy, 2022, Vol. 9 No.2,

Nurfitriah SF, Jayanti K, Putri BA, Trisnawati, T, Putri R, Oktavia SS, et al. Aktivitas Antipiretik dari Beberapa Senyawa Aktif. Jurnal Buana Farma. 2021;1(3):14-20.

Nurhamidah W, Wijaya GD, Nurshazidah S, Zulfa AN, Chaerunnisa, Solihat S, et al. Anticoagulant activities of several active compounds from medicinal plants: A review. Eur J Pharm Med Res, 2024; 11(3): 13-16.

Nurul., Dani, Sujana., Yogi, Rahman Nugraha., Zahara, Farhan., & Dadang, Muhammad Hasyim. (2021). Studi In Vivo : Efek Analgesik Ekstrak Dan Ekstrak Air Akar Pakis Tangkur (Polypodium Feei Mett). Journal of Pharmacopolium, Volume 4, No. 3, Desember 2021, 242-249.

Nurulhadi ZF, Mudrikah S, Amelia T, Valentina DP, Kurniawati I, Yuniar RR, et al. Anti-hyperuricemia activity of some medicinal plants from Karawang, West Java, Indonesia: A review. Eur J Pharm Med Res, 2024; 11(3): 9-12.

Santoso, Budi., danang, Raharjo,. Desy, Ayu. (2022). Penetapan Kadar Flavonoid dan Uji Aktivitas Antioksidan Ekstrak Etanol 70%, Fraksi N-Heksana, Etil Asetat, dan Air dari Kubis Putih dan Kubis Ungu Menggunakan Metode Frap. Cerdika: Jurnal Ilmiah Indonesia, September 2022, 2 (9), 752-764.

Setiawan MA, Fuadah TZ, Ningsih DU, Nabil M, Nababan A, Nurhabibah I, et al. The genus Begonia (Begoniaceae): A review of pharmacological studies. Eur J Biomed Pharm Sci, 2024; 11(3): 04-07.

Styawan, Anita. A., dan Budiman, Hendra. (2015). Pengaruh Penurunan Dosis Dari Ekstrak Batang Brotowali (Tinospora Crispa, L.) Terhadap Efek Antipiretik Pada Tikus Putih Jantan Galur Wistar. Cerata Journal Of Pharmacy Science.

Tamimi, Alyah Ananta Pratiwi., Edwin, de Queljoe., Jainer, Pasca Siampa.(2020). Uji Efek Analgesik Ekstrak Etanol Daun Kelor (Moringa Oleifera Lam.) Pada Tikus Putih Jantan Galur Wistar (Rattus Norvegicus). Manado : PHARMACON- FMIPA Universitas Sam Ratulangi.

Ujung RMU, Khoerunnisa A, Abimanyu ARP, Amalia, Rahayu ADP, Mangunsong DT, et al. Anti-diarrheal activity of some medicinal plants in Indonesia: A review. Eur J Biomed Pharm Sci, 2024; 11(3): 11-14.

Putri A, Putri AA, Nurhalisa E, Nurazizah F, Labibah HT, Iskandar JD, et al. Apium graveolens for the treatment of cardiovascular disease: A review. Eur J Biomed Pharm Sci, 2024; 11(3): 19-23.

Zheng, When-Jun., Ying-Shan, Ren., Miao-Li, Wu., Ya-Ling, Yang., Yi, Fan e., Xiu- Hong, Piao., Yue-Wei, Ge., & Shu-Mei, Wang. (2021). A review of the traditional uses, phytochemistry and biological activities of the Melastoma genus. Journal of Ethnopharmacology. https://doi.org/10.1016/j.jep.2020.11332.

Zulkarnaen P, Fadila N, Fadhilah LN, Kartika, Kardila K, Laely N, et al. Pharmacological activity of Selaginella doederleinii Hieron: An updated review. Eur J Pharm Med Res, 2024; 11(3): 17-20.