UJI AKTIVITAS ANTIINFLAMASI EKSTRAK BATANG AKAR KUNING (Arcangelisia flava (L.) Merr.) SECARA IN VITRO
Abstract
Inflammation is a natural response that occurs in tissue damage caused by oxidative stress. Yellow root plant is one of the plants from the Menispermaceae family that contains bioactive compounds with several potential pharmacological activities, including as anti-inflammatory. The purpose of this study was to test the effect of administration of n-hexane, ethyl acetate, and 70% ethanol extract of yellow root on anti-inflammatory activity and test the most optimal anti-inflammatory activity on the type of solvent of yellow root extract (Arcangelisia flava (L.) Merr.). This study used a method of stabilizing red blood cell membranes in vitro which was measured using a UV-Vis spectrophotometer. Stabilization was observed through the inhibitory ability of yellow root extract against red blood cell lysis due to the induction of a hypotonic solution which was then compared with a positive control (Ibuprofen). Results showed that n-hexane, ethyl acetate, and 70% ethanol extracts of yellow root can stabilize red blood cell membranes. Data analysis showed significant significance that there was a significant percent difference in red blood cell membrane stabilization in the four test groups. The most optimal anti-inflammatory activity was found in yellow root n-hexane extract at a concentration of 250 ppm which was 48.76% comparable to the positive control (Ibuprofen) at a concentration of 50 ppm of 59.09%.
References
Akmalia, R. A., Hajrah Hajrah, & Rijai, L. (2016). Aktivitas Antiinflamasi Ekstrak Rimpang Temu Kunci (Boesenbergia pandurata) Secara In-Vitro. In Proceeding of Mulawarman Pharmaceuticals Conferences, 4, 289–294.
Alkandahri MY, PN Siahaan, Salim E, Fatimah C. AntiInflammatory activity of Cep-cepan leaves (Castanopsis costata (Blume) A.DC). Int J Curr Med Sci. 2018;8(4A):424-429.
Amalia A, Romdon DS, Kusumawati N, Salsabila MA, Afifah ND, Shaomitha R, et al. Antihyperuricemia activities of several active compounds from medicinal plants: A review. Eur J Biomed Pharm Sci, 2024; 11(3): 15-18.
Anggraeni, E. D. (2021). Uji Aktivitas Aniinflamasi Ekstrak Akar Kuning (Arcangelisia flaca (L.) Merr.) Pada Mencit Jantan Galur Swiss Webster. Al-Ghifari University.
Aprilianti, R. G., Permataningtyas, T., & Wardana, F. Y. (2022). Oral Subchronic Toxicity Test of Yellow Root Ethanol Extract (Arcangelisia flava Merr.) In Rats (Mus musculus). Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), 8(2), 154–161. https://doi.org/10.22487/j24428744.2022.v8.i2.15961
Askandari. (2015). Uji Aktivitas Anti Inflamasi Ekstrak Etanol 70% Buah Parijoto (Medinila Speciosa Blume) Secara In Vitro Dengan Metode Stabilisasi Membran Hrbc (Human Red Blood Cell). Universitas Islam Negeri Syarif Hidayatullah.
Budiyanti LE, Saputra MYKA, Wulandari SA, Amallia S, Azzahra AJ, Sagala BC, et al. Antihypertensive effects of some medicinal plants in Indonesia: A review. Eur J Pharm Med Res, 2024; 11(3): 5-8.
Dewi, B. A., Setianto, R., & Rosita, F. (2020). Uji Aktivitas Tanaman Pangotan (Microsorium beurgerianum (Miq.) Ching) Sebagai Antiinflamasi Secara Invitro dengan Metode HRBC (Human Red Blood Cell). Jurnal Ilmiah Kesehatan, 1(2), 15–20.
Gandjar, I. G., & Rohman, A. (2007). Kimia Farmasi Analisis. Pustaka Pelajar. .
Harborne, J. B. (1998). Phytochemical Methods (3rd ed.). Chapman and Hall.
Hardi, R. S., Slamet, S., & Kamilla, L. (2019). Uji Aktivitas Anti Inflamasi Ekstrak Bawang Dayak (Eleutherine Americana L. Merr) terhadap Stabilisasi Membran Sel Darah Merah. Jurnal Laboratorium Khatulistiwa, 2(1), 30. https://doi.org/10.30602/jlk.v2i1.324
Ihsan, H., Pratama, I. S., & Hanifa, N. I. (2021). Uji Aktivitas Antiinflamasi Infusa Bunga Pukul Empat Secara In Vitro. Acta Pharmaciae Indonesia: Acta Pharm Indo, 9(1), 21.
Ilyas, M. Y., Saehu, M. S., Ertin, E., Irma, I., & Nurhikma, N. (2022). Antiinflammatory Effects of Fraction From Galing Stem Ethanol Extract (Cayratia Trifolia L. Domin) In Vitro. Jurnal Farmasi Sains Dan Praktis, 7(3), 289–297. https://doi.org/10.31603/pharmacy.v7i3.6107
Jung, H. A., Jin, S. E., Ahn, B. R., Lee, C. M., & Choi, J. S. (2013). Anti-inflammatory activity of edible brown alga Eisenia bicyclis and its constituents fucosterol and phlorotannins in LPS-stimulated RAW264.7 macrophages. Food and Chemical Toxicology, 59, 199–206. https://doi.org/10.1016/j.fct.2013.05.061
Luliana, S., Susanti, R., & Agustina, E. (2017). Uji aktivitas antiinflamasi ekstrak air herba ciplukan (Physalis angulata L.) terhadap tikus putih (Rattus norvegicus L.) jantan galur wistar yang diinduksi karagenan. Traditional Medicine Journal, 22(3), 199–205.
Mediansyah, A., & Rahmanisa, S. (2017). Hubungan Ibuprofen terhadap Ulkus Gaster. Jurnal Majority, 6(1), 6–10.
Nurulhadi ZF, Mudrikah S, Amelia T, Valentina DP, Kurniawati I, Yuniar RR, et al. Anti-hyperuricemia activity of some medicinal plants from Karawang, West Java, Indonesia: A review. Eur J Pharm Med Res, 2024; 11(3): 9-12.
Oyedapo, O. O., Akinpelu, B. A., Akinwunmi, K. F., Adeyinka, M. O., & Sipeolu, F. O. (2010). Red blood cell membrane stabilizing potentials of extracts of Lantana camara and its fractions. International Journal of Plant Physiology and Biochemistry, 2(4), 46–51.
Poncowati, S., Soenardjo, N., Taufiq-Spj, N., & Sibero, M. T. (2022). Profil Senyawa Metabolit Sekunder Ekstrak Daun Mangrove Lumnitzera racemosa Asal Perairan Teluk awur, Jepara. Journal of Marine Research, 11(4), 794–804. https://doi.org/10.14710/jmr.v11i4.34325
Pratiwi, R., & Harlia, M. A. W. (2017). Aktivitas Antiinflamasi dan Toksisitas Dari Ekstrak Daun Nanas Kerang (Rhoeo Discolor). Jurnal Kimia Khatulistiwa, 6(2).
Rachmawati A, R., Wisaniyasa, N. W., & Suter, I. K. (2020). Pengaruh Jenis Pelarut Terhadap Aktivitas Antioksidan Ekstrak Meniran (Phyllanthus Niruri L.). Jurnal Ilmu Dan Teknologi Pangan (ITEPA), 9(4), 458.
Reilkoff, R. A., Bucala, R., & Herzog, E. L. (2011). Fibrocytes: emerging effector cells in chronic inflammation. Nature Reviews Immunology, 11(6), 427–435.
Sukmawati, S., Wati, A., & Muflihunna, A. (2022). Uji Aktivitas Ekstrak Kombinasi Rimpang Kunyit (Curcuma domestica Val.) dan Kurma (Phoenix dactylifera L) sebagai Antiinflamasi Secara In Vitro. Window of Health: Jurnal Kesehatan, 735–744.
Tavita, G. E., Lestari, D., Linda, R., Apindiati, R. K., & Rafdinal, R. (2022). Phytochemical Testing and In Vitro Anti-inflammatory Activity on Ethanol Extract of Akar Kuning (Arcangelisia flava L) Stems from West Kalimantan. Jurnal Biologi Tropis, 22(4), 1334–1339.
Wiranto, E., Wibowo, M. A., & Ardiningsih, P. (2016). Aktivitas antiinflamasi secara in-vitro ekstrak teripang butoh keling (Holothuria leucospilota Brandt) dari pulau lemukutan. Jurnal Kimia Khatulistiwa, 5(1).
Zulkarnaen P, Fadila N, Fadhilah LN, Kartika, Kardila K, Laely N, et al. Pharmacological activity of Selaginella doederleinii Hieron: An updated review. Eur J Pharm Med Res, 2024; 11(3): 17-20.
