AKTIVITAS ANTIBAKTERI KOMBINASI EKSTRAK DAUN NILAM (Pogostemon cablin Benth.) DAN DAUN KEMANGI (Ocimum basilicum L.) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus

Elvie Rifke Rindengan; Sri Handayani Gurning; Marcelia Paputungan; Selfie Petronela Joice Ulaen; Rilyn Novita Maramis

doi:10.36805/jbf.v5i3.1503

Elvie Rifke Rindengan Poltekkes Kemenkes Manado, Manado, Indonesia
Sri Handayani Gurning Poltekkes Kemenkes Manado, Manado, Indonesia
Marcelia Paputungan Poltekkes Kemenkes Manado, Manado, Indonesia
Selfie Petronela Joice Ulaen Poltekkes Kemenkes Manado, Manado, Indonesia
Rilyn Novita Maramis Poltekkes Kemenkes Manado, Manado, Indonesia

DOI: https://doi.org/10.36805/jbf.v5i3.1503

Keywords: Antibacterial, Extract, Basil Leaf, Patchouli Leaf, Staphylococcus aureus

Abstract

Infections caused by Staphylococcus aureus bacteria can lead to various health problems, including skin infections that result in redness, swelling, blisters, and pus-filled lumps. The use of herbal plants is an alternative treatment for infections. Plants known to have antibacterial potential include patchouli leaves (Pogostemon cablin Benth.) and basil leaves (Ocimum basilicum L.). This study aims to determine the antibacterial activity of a combination of patchouli leaf extract (Pogostemon cablin Benth.) and basil leaves (Ocimum basilicum L.) against the growth of Staphylococcus aureus bacteria. The type of research used is a laboratory experiment. Extraction of patchouli leaves and basil leaves was performed using the maceration method with 96% ethanol. The test solution was prepared in three concentrations: 10%, 15%, and 20%. The combination was made by mixing each concentration in 1:1 ratio. The antibacterial activity test was conducted using the disc diffusion method, with a positive control consisting of erythromycin discs and a negative control of a 1% Tween 80 solution. Observations were made for 24 hours, then the inhibition zone around the paper disc was measured using a caliper. The combination of patchouli and basil leaf extracts can inhibit the growth of Staphylococcus aureus bacteria.

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