SKRINING FITOKIMIA DARI EKSTRAK DAUN KANGKUNG PAGAR (Ipomoea carnea Jacq) DAN UJI ANTIBAKTERI TERHADAP Bacillus subtilis
Abstract
Kangkung Pagar (Ipomoea carnea Jacq) leaves is one of the plants that is used as a treatment. This study aims to determine the content of secondary metabolites and antibacterial activity of kangkong pagar leaves against Bacillus subtilis. Extraction using maceration method with n-hexane, ethyl acetat and methanol. Antibacterial assay using well diffusion method. The extract concentrations used were 40%, 60%, 80% and 100%. The positive control used was Ciprofloxacin and the negative control used was DMSO 5%. Data were analyzed by one way ANOVA test followed by Post Hoc Tukey test. The results showed that the extracts of ethyl acetate, methanol and n-hexane had a minimum inhibitory concentration of 40% with inhibition zones formed of 8.23 mm, 9.4 mm and 4.2 mm. Meanwhile, at the highest concentration of 100%, an inhibition zone of 12.6 mm was formed in the ethyl acetate extract, 15.23 mm in the methanol extract and 11.4 mm in the n-hexane extract. The results showed that extract of Kangkung Pagar (Ipomoea carnea Jacq)leaves was able to inhibit the growth of Bacillus subtilis bacteria.
References
Abriyani, E., Fikayuniar, L., dan Safitri, F. Skrining Fitokimia Dan Bioaktivitas Antioksidan Ekstrak Metanol Bunga Kangkung Pagar (Ipomoea Carnea Jack.) Dengan Metode Dpph (2, 2-Difenil-1-Pikrilhidrazil). Jurnal Sains dan Ilmu Farmasi. 2021. Vol 6(1), 32-42.
Ergina., Nuryanti, S., dan Pursitasari, I. D. Uji Kualitatif Senyawa Metabolit Sekunder Pada Daun Palado (Agave Angustifolia) Yang Diekstraksi Dengan Pelarut Air Dan Etanol. Jurnal Akademika Kimia. 2014. Vol. 3(3): 165-172.
Fatima, N., Rahman, MY., Khan, Md. A., dan Fu, J. A review on Ipomoea carnea: Pharmacology, toxicology and phytochemistry. Journal of Complementary and Integrative Medicine. 2014. 11(2): 55-62.
Fitriani, A., Setiyorini, E., dan Khanifah, F. Efektifitas Ekstrak Daun Srikaya (Annona squamosa L) Terhadap Pertumbuhan Staphyloccoccus aureus Secara In Vitro. 2017. Jurnal Insan Cendekia. Vol 6 No. 1
Harbone, J. B. (1996). Metode Fitokimia penuntun cara modern menganalisis tumbuhan. Bandung: Penerbit ITB Bandung.
Hatmanti, A. Pengenalan Bacillus SPP. Oseana Volume XXV, Nomor 1, 2000 : 31-41
Jain, N. M., Jha, V., Shrivastava, N., Sihare, V., dan Jain, A. An Experimental Evaluation Of Ipomoea Carnea Leaves Extract As Anti-Furuncle Modality: A Preliminary Study. Indo American Journal of Pharmaceutical Research. 2017. Vol. 7 (8).
Kamal, A.M., Shakour, Z. T. B., All, S. R. A., Sleem, A. A., dan Haggag, E. G. Phytochemical and Biological Investigation of Ipomoea carnea Jacq. Grown in Egypt. International Journal of Pharmacognosy and Phytochemical Research. 2017. Vol. 9(2); 266-281.
Leonita, S., Bintang, M., Pasaribu, F. H. Isolation and Identification of Endophytic Bacteria from Ficus variegata Blume as Antibacterial Compounds Producer. 2015 Vol 2 (3): 116 - 128
Mayasari, U dan Berutu, AV. Uji Aktivitas Ekstrak Daun Pirdot (Saurauia Vulcani Korth) Terhadap Pertumbuhan Bakteri Bacillus Subtilis. Klorofil: Jurnal Ilmu Biologi dan Terapan. 2020. Vol 4(1): 1-5.
Nuraeni, E., Alkandahri, MY., Tanuwidjaja, SM., Fadhilah, KN., Kurnia, GS., Indah, D., et al. Ethnopharmacological Study of Medicinal Plants in the Rawamerta Region Karawang, West Java, Indonesia. Open Access Macedonian Journal of Medical Sciences. 2022; 10(A): 1560-1564.
Wahyuningsih ES, Puspitasari M, Gunarti NS, Alkandahri MY. Uji Aktivitas Antibakteri Face Mist Ekstrak Etanol Daun Andong Merah (Cordyline fruticosa (L) A. Chev.) Terhadap Propionibacterium acnes. Pharma Xplore: Jurnal Sains dan Ilmu Farmasi. 2023;8(2):104-127
