Jurnal Buana Farma

AKTIVITAS ANTIJAMUR EKSTRAK ETANOL DAUN KARI TERHADAP Candida albicans ATCC 14053

Putri Eka Sari, Pra Panca Bayu Chandra, Nia Lisnawati, Neng Ami — Mon, 30 Jun 2025 16:20:33 +0000

Medicinal plants are recognized as valuable sources of secondary metabolites with therapeutic potential. Candidiasis caused by Candida albicans is a common health problem in tropical regions. The prolonged use of synthetic antifungal agents may lead to resistance, highlighting the need for effective natural alternatives. Curry leaves (Murraya koenigii (L) Spreng) are known to contain flavonoids and alkaloids with potential antifungal activity. This study aimed to evaluate the antifungal activity of the 96% ethanol extract of curry leaves against Candida albicans ATCC 14503. The extract was prepared by maceration using 96% ethanol as the solvent. Phytochemical screening revealed the presence of flavonoids and saponins. The antifungal activity was tested using the disc diffusion method at concentrations of 6,25%, 12,5%, 25%, and 50%. Ketoconazole was used as positive control, while 96% ethanol served as a negative control. The test was performed in triplicate, and the inhibition zones were measured and averaged. The results showed that the 96% ethanol extract of curry leaves at all tested concentrations did not exhibited antifungal activity against C. albicans as indicated by the absence of inhibition zones on the media. It can be concluded that the extract, under the given conditions, was not effective in inhibiting fungal growth. This suggests that the extraction method and tested concentrations may not have been sufficient to isolate or concentrate the active compounds. Further studies using alternative extraction techniques, higher concentrations or isolated compounds are needed to better evaluate the antifungal potential of M. koenigii.

POTENSI ANTIINFLAMASI SENYAWA BIOAKTIF DARI SYZYGIUM MYRTIFOLIUM: STUDI MOLECULAR DOCKING BERTARGET COX-2

Mon, 30 Jun 2025 16:23:06 +0000

Chronic inflammation contributes significantly to various degenerative diseases, with Cyclooxygenase-2 (COX-2) being a central target for anti-inflammatory drug development. Syzygium myrtifolium, a native Indonesian plant, is known to contain a variety of bioactive compounds with pharmacological potential. This study aims to investigate the anti-inflammatory activity of S. myrtifolium active compounds on COX-2 using a molecular docking approach. The tested compounds include avicularin, betulinic acid, ursolic acid, and two flavanones modeled and docked against the COX-2 protein (PDB ID: 5F19) using YASARA. Docking simulations were performed using YASARA software via a blind docking method and visualized through Discovery Studio. The results revealed that four compounds demonstrated stronger binding affinity than celecoxib, with (2S)-7-Hydroxy-5-methoxy-6,8-dimethylflavanone showing the most stable interaction with 9.0450 kcal/mol binding energy. Toxicity prediction suggests these compounds are generally safe based on LD50 values and toxicity class. These findings support the potential of S. myrtifolium as a natural source of COX-2 inhibitors.

UJI KUALITATIF BAHAN PENYUSUN SEDIAAN MIKROSFER BSA DENGAN KITOSAN DAN NA-TPP

Rifka Anggraini Anggai, Faradila Ratu Cindana Mo’o — Mon, 30 Jun 2025 16:25:12 +0000

This study aims to evaluate the qualitative characteristics of the components used in a protein delivery system based on microspheres, namely Bovine Serum Albumin (BSA), chitosan, sodium tripolyphosphate (Na-TPP), and maltodextrin, through organoleptic examination, Fourier Transform Infrared Spectroscopy (FTIR), and Differential Thermal Analysis (DTA). Organoleptic observations confirmed that all materials met standard physical characteristics as described in recent literature. FTIR spectra of BSA revealed the presence of amide I and II bands, indicating the preservation of its secondary protein structure. Chitosan exhibited functional groups capable of forming ionic crosslinks with TPP, essential in microsphere formation. The FTIR spectrum of TPP showed characteristic P=O and P–O–P absorption bands, confirming its role in ionic gelation. Maltodextrin displayed hydroxyl and carbonyl groups, supporting its function as a stabilizer in the formulation. DTA results demonstrated that all materials possess adequate thermal stability to withstand the formulation process. These findings suggest that the combination of BSA, chitosan, Na-TPP, and maltodextrin is compatible and effective in developing a stable microsphere-based protein delivery system, both in terms of chemical structure and thermal behavior.

SURVEI ETNOFARMAKOGNOSI DAN PROFIL KIMIA DARI 33 TUMBUHAN OBAT DI PURWAKARTA, INDONESIA

Lia Fikayuniar, Ermi Abriyani, Dewi Yunita — Mon, 30 Jun 2025 16:28:42 +0000

Traditional medicine practices in Indonesia have long utilised medicinal plants as the main foundation for public health resilience. This research aims to document local knowledge of medicinal plant usage and analyse its chemical profile to strengthen the scientific validity of traditional utilisation by the Purwakarta community. The methods used include ethnopharmacognosy surveys through semi-structured interviews with snowball sampling technique, specimen collection and identification, phytochemical screening of six groups of bioactive compounds, and qualitative-quantitative data analysis (UV, FUV, RFC, FL). The results show that there are 33 species of medicinal plants used by the communities of Taringgul Tonggoh and Wanasari villages, with leaves being the most utilised part (91% of oral usage). The Zingiberaceae family dominates the utilisation, while flavonoids and tannins were detected in the highest amounts in the phytochemical screening, supporting empirical claims as antioxidants, anti-inflammatory, and antimicrobial agents. These findings demonstrate the consistency between traditional practices and pharmacological potential, while also filling the gap in ethnopharmacognosy documentation in Purwakarta. The implications of this study strengthen the scientific basis for integrating local knowledge into modern pharmacy and open up opportunities for the development of phytopharmaceuticals based on local biodiversity.

STUDI PELAPORAN KEJADIAN NYARIS CEDERA (KNC) DI INSTALASI FARMASI RAWAT JALAN RUMAH SAKIT DI KARAWANG TAHUN 2017

Dewi Darwati Agustini, Maida Safitri, Iyam Mariam — Mon, 30 Jun 2025 16:30:26 +0000

Pelaporan keselamatan pasien pada pelayanan kefarmasian dititikberatkan pada KNC yang menjadi aspek penting dalam menunjang keselamatan pasiend engan mengurangi terjadinya medication error. Penelitian ini bertujuan untuk mengetahui banyaknya angka kejadian nyaris cedera yang dilaporkan selama tahun 2017 dan pada bagian mana paling sering terjadinya KNC di unit rawat jalan RSUD Karawang. Penelitian dilakukan bersifat deskriptif cross sectional. Penelitian dilakukan pada lembar rekapitulasi laporan KNC selama tahun 2017. Hasil penelitian menunjukkan selama tahun 2017 terjadinya KNC pada penginputan resep sebanyak 316 kali, penulisan etiket 47 kali, peracikan 122 kali, dan penyerahan obat sebanyak 4 kali. Total keseluruhan terjadi KNC sebanyak 489 kali atau sekitar 0,5% terhadap lembar resep. Dapat disimpulkan bahwa selama tahun 2017 bagian yang paling sering mengalami KNC adalah pada penginputan resep sebanyak 316 kali terjadinya KNC.

PENGARUH PEMBERIAN ANTIBIOTIK PROFILAKSIS TERHADAP LUARAN BEDAH PADA PASIEN GERIATRI: SISTEMATIK REVIEW

Ricky Aditya Syam — Mon, 30 Jun 2025 16:32:44 +0000

This systematic review examined the effectiveness of prophylactic antibiotic administration in reducing the risk of surgical wound infection in geriatric patients. This topic is of particular importance globally given the rapid increase in the elderly population susceptible to postoperative complications, as well as the variability of the existing evidence regarding the effectiveness and optimal duration of prophylactic antibiotics. Although many studies have been conducted, there has not been a comprehensive synthesis of recent evidence to fill the gap. This study, which is a systematic review based on the PRISMA 2020 guidelines, analyzes articles from the Scopus, PubMed, and Google Scholar databases (2020-2025). The study involved 31 articles that met the inclusion criteria, including retrospective observational studies and randomized clinical trials, with the primary outcomes of postoperative infections and antibiotic-related side effects. The analysis method includes evaluation of the quality of the study as well as narrative and quantitative synthesis. The results showed that the administration of prophylactic antibiotics appropriately significantly reduced the risk of infection, especially in high-risk surgeries such as colorectal, orthopedic, and cardiothoracic, with an optimal duration of about 24 hours postoperatively. The risk of antibiotic resistance and opportunistic infections remains a major concern. The practical implications demand the development of evidence-based guidelines with adjustments to the duration of administration and the strengthening of alternative prevention strategies. Advanced research with a multicenter randomized clinical trial design is essential to optimize prophylactic regimens in diverse and complex elderly populations.

UJI ANTIBAKTERI EKSTRAK BATANG GAHARU (Aquilaria malaccensis) BERBASIS KOLIN KLORIDA:ASAM SITRAT TERHADAP Bacillus cereus dan Shigella dysenteriae

Mon, 30 Jun 2025 16:34:25 +0000

Infections caused by Bacillus cereus and Shigella dysenteriae are serious health problems as they can lead to gastrointestinal disorders and contribute to the emergence of antibiotic resistance. One potential strategy to overcome this challenge is the discovery of new antibacterial agents derived from natural sources, such as agarwood (Aquilaria malaccensis). This study aimed to evaluate the antibacterial activity of gaharu stem extract using a Natural Deep Eutectic Solvent (NADES) composed of choline chloride and citric acid through the Ultrasound Assisted Extraction (UAE) method. The antibacterial assay was performed using the disc diffusion method with various NADES ratios (1:1, 2:1, 1:2, 1:3, 1:4, and 4:1 g/g), with chloramphenicol as the positive control and 10% DMSO as the negative control. The results indicated that the NADES extract with a 1:4 g/g ratio produced the largest inhibition zones against Bacillus cereus and Shigella dysenteriae. Moreover, the Minimum Inhibitory Concentration (MIC) test showed that the 1:4 NADES extract effectively inhibited bacterial growth at a concentration of 30%. These findings suggest that gaharu stem extract prepared with NADES is a promising, eco-friendly, and potent antibacterial agent.

ARTIKEL REVIEW : AKTIVITAS ANTIOKSIDAN BUNGA MAWAR MERAH (Rosa damascene Mill.)

Dian Ayu Oktavia, Bella Fevi Aristia, Kadeq Novita Prajawanti — Mon, 30 Jun 2025 16:36:09 +0000

Red rose (Rosa damascena Mill.) is an ornamental plant that is rich in bioactive compounds such as flavonoids, anthocyanins, phenols, vitamin C, tannins, and saponins that function as natural antioxidants. The purpose of this article is to review research results related to the antioxidant activity of red rose flowers in various dosage forms and extraction methods. This review was conducted through a literature review of 10 selected scientific articles in the range of 2015-2025 using the exclusion and inclusion method. The results showed that most of the red rose flower extracts showed very strong antioxidant activity, characterized by IC₅₀ values < 50 ppm, even in some studies reaching < 20 µg/mL. Antioxidant activity is influenced by the type of solvent, temperature and processing time, as well as the combination of ingredients such as bengkoang or mint leaves. Polar extracts such as ethanol and methanol tend to give the best results, while high temperature treatment and long heating times tend to reduce the effectiveness of active compounds. Thus, rose red flowers have great potential as a source of natural antioxidants that can be utilized in functional food, cosmetic, and pharmaceutical products.

EKSPLORASI AKTIVITAS HEPATOPROTEKTIF EKSTRAK ETANOL TEMU KUNCI (Boesenbergia rotunda) MELALUI PARAMETER MAKROPATOLOGI ORGAN HATI DAN INDEKS ORGAN PADA MODEL HEPATOTOKSISITAS TIKUS

Mawandha Sari Harahap, Heppy Nova Jayanti, Desni Rinanda Silitonga, Candrika — Mon, 30 Jun 2025 16:40:09 +0000

Hati merupakan organ paling berperan dalam mayoritas sistem metabolisme pada tubuh. Metabolisme N-acetyl-p-aminophenol (APAP) memiliki metabolit reaktif N-acetyl-p-benzoquinone imine (NAPQI) yang dapat menyebabkan berkurangnya antioksidan glutation hati serta disfungsi mitokondria dan mengakibatkan meningkatnya jumlah radikal bebas dihati, yang pada akhirnya akan merusakkan hati. Antioksidan dibutuhkan guna meminimalisir radikal bebas. Rimpang temu kunci (Boesenbergia rotunda (L.) Mansf) mengandung metabolit sekunder berupa flavonoid yang diketahui memiliki sifat antioksidan sehingga rimpang temu kunci memiliki potensi untuk melindungi hati dari kerusakkan. Penelitian ini bertujuan untuk menganalisis aktivitas hepatoprotektif ekstrak etanol rimpang temu kunci terhadap tikus putih jantan yang diinduksi dengan APAP.

Tikus putih jantan sebanyak 24 ekor digunakan pada penelitian ini, tikus dibagi dalam 6 kelompok perlakuan yakni kelompok kontrol normal, kontrol negatif diberi CMC-Na 0,5%, kelompok uji diberi ekstrak etanol rimpang temu kunci (EERTK) dosis 250, 500 serta 750 mg/kgBB dan kelompok kontrol positif diberi asetilsistein 200 mg. Selama 10 hari berturut-turut. Pada hari kesepuluh diinduksi dengan APAP 800 mg/kgBB secara oral. Setelah 16 jam, dilakukan pembedahan dan pengambilan organ hati. Organ hati untuk pengukuran indeks relatif organ dan makropatologi organ hati. Data dianalisis menggunakan one way-ANOVA lalu dilanjutkan Post Hoc Turkey HSD. Hasil analisis menjelaskan bahwa pretreatmen dengan EERTK secara signifikan menyebabkan indeks relatif organ hati menurun akibat dari pemberian EERTK, disisi lain EERTK juga memperbaiki makropatologi organ hati.

LITERATURE REVIEW: POTENSI OBAT BAHAN ALAM DARI TANAMAN SEMANGGI AIR (Marsilea crenata)

Pra Panca Bayu Chandra, Dian Ratih Laksmitawati — Mon, 30 Jun 2025 16:42:18 +0000

The use of plants as natural medicine is used because Indonesia has a wealth of natural resources. One of the plants used is water clover (Marsilea crenata). This plant is used as food and medicine. This study aims to identify the potential of natural medicine from water clover (Marsilea crenata). The research method in the literature study is to apply journal searches to the Google Scholar, Science Direct, and Pubmed databases with the keywords water clover (Marsilea crenata) and pharmacological activity. The journals used are national and international journals published from 2012 to 2025. The journals used are research on the potential of water clover as a natural medicine in silico, in vitro and in vivo. From the results of the literature study, 9 journals were obtained. After obtaining research data from journals, they were then combined and studied, then explained and explained systematically. The results of the study showed that water clover leaves in both extract and fraction forms have pharmacological activities including anticholesterolemia, antihyperglycemia, antioxidant, anti-inflammatory, HMG-CoA reductase enzyme inhibitory activity, sperm quality improvement activity in hyperglycemic mice, anti-neuroinflammatory serum. The content of secondary metabolites found in Marsilea crenata is a bioactive compound that is responsible for providing pharmacological activity.

STUDI KANDUNGAN SENYAWA KIMIA TUMBUHAN OBAT DI DESA LEGOKHUNI DAN SUKADAMI KABUPATEN PURWAKARTA

Ermi Abriyani, Lia Fikayuniar, Amanda Auliya Zulfa — Mon, 30 Jun 2025 16:44:22 +0000

A study on the chemical compound content of medicinal palnts was conducted in Legokhunimand Sukadami Villagesa, Purwakarta Region. The aim of this study was to examine the chemical compound found in various plants used as traditional medicine in Legokhuni and sukadami Villages in Purwakarta Region. The method used involved interviewing several residents from both villages regarding the traditional medicines they used, and performing phytochemical screening assay using specific reagents. The results of this study showed there 33 types of medicinal plants identified through interviews. The phytochemical screening revealedthat the most commonly found secondary metabolites in these palnt were alkaloids and flavonoids, each present in all33 palnt species. Quinoneswere found in 24 plant species, phenolics and tannins in 26 species, and saponins in 30 species. These plant types are therefore considered promising candidates for further in-dept research.

AKTIVITAS ANTICAPLAK SEDIAAN SPRAY YANG MENGANDUNG MINYAK ROSEMARY,BERGAMOT DAN CUKA APEL

Affan Aufar Ath-Thaariq, Bayu Febram Prasetyo, Supriyono — Mon, 30 Jun 2025 16:45:49 +0000

Apple cider vinegar and rosemary oil have an acaricidal effect on R. sanguineus ticks. Both ingredients offer health benefits. This study aimed to observe the effect of a spray preparation containing apple cider vinegar and rosemary oil at various concentrations on the mortality of R. sanguineus ticks. The study was conducted from February 2025 to April 2025. It used 75 R. sanguineus ticks, divided into 5 groups, with each group having three repetitions. K1, the negative control, was treated with pure distilled water. K2, the positive control, was treated with Fipronil 3% (Frontline brand). K3 was given bergamot oil 5%, rosemary oil 10%, and apple cider vinegar 5%. K4 was given bergamot oil 5%, rosemary oil 10%, and apple cider vinegar 10%. K5 was given bergamot oil 5%, rosemary oil 10%, and apple cider vinegar 15%. Each treatment group consisted of 5 ticks. Observations were made at 30, 60, 90, 120, and 180 minutes post-contact. The results showed that the group treated with bergamot oil 5%, rosemary oil 10%, and apple cider vinegar 15% achieved a Lethal Time 50 (LT50) at 106 minutes. The conclusion indicates that apple cider vinegar at a concentration of 15% required the least amount of time to kill the ticks

ANALISIS RASIONALITAS OBAT DIABETES MELITUS TIPE II DI UPT PUSKESMAS SAKETI PERIODE JULI-DESEMBER 2023

Santi Endrawati Santi, Sariyati Maimunah, Andi Cahyadi — Mon, 30 Jun 2025 16:49:02 +0000

Diabetes Mellitus (DM) is a group of metabolic diseases characterized by chronic hyperglycemia, the treatment process must be carried out rationally, including correct diagnosis, correct patient, correct drug, correct dose, correct indication and correct time interval. This study aims to determine the rationality of the use of antidiabetic drugs at the Saketi Health Center UPT in the period July-December 2023. Retrospective data collection based on medical records using the Total sampling method was carried out on 138 samples that had met the inclusion and exclusion criteria. Data analysis using univariate analysis using the Microsoft Office Excel 2010 program, data was analyzed using the 2021 Perkeni literature and the Indonesian Ministry of Health 2019 for the rationality of 138 Diabetes Mellitus (DM) patients. Patient characteristics in the form of gender, there is a percentage of women 99 (72%) and men 39 (28%), Diabetes Mellitus (DM) which often occurs in the age range of 46-65 years with 110 cases (80%) The percentage of rationality of the use of diabetes mellitus drugs in patients with the right diagnosis is 138 patients 100%, right patients 138 100%, right drug selection 26 (19%) patients, wrong drug selection 112 (81%), right indication 138 patients 100%, right dose patients 138 100% and interval time of administration 138 patients.

SENYAWA ALAMI DAUN BAWANG UNTUK DIABETES: PENDEKATAN IN SILICO TERHADAP PPAR-γ

Himyatul Hidayah, Ruswanto, Erisa Mindawati , Surya Amal, Farhamzah — Sat, 02 Aug 2025 01:34:13 +0000

Leek leaves (Allium fistulosum L.) are plants that contain flavonoid compounds with diverse biological activity potential. This study aims to examine the stable interaction of the docking results between the PPAR-γ receptor complex structure and the best candidate flavonoid compounds in leek leaves as antidiabetic candidates. The method used in this study is quantitative and designed using molecular docking techniques based on computational molecular dynamics (MD), which simulates the dynamic behaviour of a molecular system as a function of time, treating each entity in the simulation box (protein, ligand, or water, if present) as flexible. The results of molecular docking on 16 onion leaf flavonoid compounds showed that hesperidin had the smallest Gibbs free energy (ΔG) of -10.0 kcal/mol, which is close to that of natural ligands. The RMSD and RMSF values are sufficiently stable. Hesperidin also has good pharmacokinetic properties and is non-toxic, thus meeting the criteria for drug-likeness as a potential oral drug candidate. These findings indicate the potential for developing flavonoid compounds, particularly hesperidin from onion leaves, as a candidate for an antidiabetic drug by inhibiting the PPARγ receptor. This study provides a foundation for further in vitro and in vivo studies to validate its biological activity.

FORMULASI DAN UJI AKTIVITAS ANTIOKSIDAN SEDIAAN FACE MIST FRAKSI DAUN LIDAH MERTUA (Sansevieria trifasciata Prain)

Nia Yuniarsih, Farhamzah farhamzah, Nur Intan Wulansari — Sun, 10 Aug 2025 02:29:05 +0000

Face mist is a cosmetic that is often used as a skin freshener, serves to refresh facial skin by removing residual oil, as a mild disinfectant, while closing pores again and can have activity as an antioxidant. The Sansevieria trifasciata Prain contains flavonoids, alkaloids and tannins that have the potential to be a source of natural antioxidants. This study aims to examine the potential of n-hexane, ethyl acetate and water fractions of Sansevieria trifasciata Prain as active substances in face mist formulations. This study used the Fractionation and DPPH methods to determine the effectiveness of antioxidant activity of various formulations with F1 (n-hexane fraction); F2 (ethyl acetate fraction) and F3 (water fraction), which were compared with commercial products.. The results showed that F1 and F2 had strong antioxidant activity, while F3 had moderate antioxidant activity. The results of physical evaluation and stability tests using the accelerated stability method (cycling test) showed that the resulting face mist preparation met SNI standards, had good stability and did not cause irritation. From these results, it can be concluded that the n-hexane, ethyl acetate and Sansevieria trifasciata Prain leaf water fractions have the potential to be effective natural antioxidants in the formulation of face mist preparations.