PENGARUH PROSES FERMENTASI AEROB DAN ANAEROB TERHADAP KADAR FLAVONOID DAN STEROID PADA DAUN SUKUN HIJAU (Artocarpus altilis (Parkinson) Fosberg) YANG DIFERMENTASI

Authors

  • Hesti Riasari Departmen Biologi Farmasi, Sekolah Tinggi Farmasi Indonesia, Bandung, Jawa Barat, Indonesia ,
  • Wiwin Winingsih Departmen Farmakokimia, Sekolah Tinggi Farmasi Indonesia, Bandung, Jawa Barat, Indonesia. ,
  • Sri Gustini Husein Departmen Farmakokimia, Sekolah Tinggi Farmasi Indonesia, Bandung, Jawa Barat, Indonesia. ,
  • Nela Simanjuntak Departemen Farmakologi, Sekolah Tinggi Farmasi Indonesia, Bandung, Jawa Barat, Indonesia. ,

DOI:

https://doi.org/10.36805/jpx.v10i1.10080

Keywords:

Breadfruit, Anaerobic Fermentation, Aerobic fermentation, Flavonoid total content, Steroid content

Abstract

This study investigates the impact of aerobic and anaerobic fermentation processes on flavonoid and steroid levels in green breadfruit (Artocarpus altilis (Parkinson) Fosberg) fermented leaves. The research evaluated flavonoid and steroid content after fermentation periods of 5 days and 10 days under both aerobic and anaerobic conditions. Results showed that after 5 days of fermentation, the flavonoid content was 0.729 under aerobic conditions and 0.825 under anaerobic conditions. Additionally, after 10 days of fermentation, the flavonoid content decreased to 0.605 under aerobic conditions and 0.664 under anaerobic conditions. Regarding steroid content, after 5 days of fermentation, aerobic conditions yielded 11.425 while anaerobic conditions produced 10.549. After 10 days, aerobic fermentation resulted in 10.861, whereas anaerobic fermentation resulted in 10.125. These findings suggest that both the duration and the type of fermentation process significantly influence the levels of flavonoids and steroids in fermented breadfruit leaves.

References

Ayala, A., Muñoz, M. F., and Argüelles, S. Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxidative Medicine and Cellular Longevity, 2014, 2014, 360438.

Aziz, S., Viesa, R., Teruna, dan Yuda, H. 2011, Standardisasi Bahan Obat Alam, Edisi Pertama. Graha Ilmu, Yogyakarta.

Cheng, S., and Sliva, D. Ganoderma lucidum for cancer treatment: we are close but still not there. Integrative Cancer Therapies, 2015, 14(3), 249-257.

Dashora, A., Rathore, K., Raj, S., and Sharma, K. Synthesis of silver nanoparticles employing Polyalthia longifolia leaf extract and their in vitro antifungal activity against phytopathogen. Biochemistry and Biophysics Reports, 2022, 31, 101320.

Dias, M.C., Pinto, D.C.G.A., and Silva, A.M.S. Plant flavonoids: Chemical characteristics and biological activity. Molecules, 2021, 26(17), 5377.

Ditjen POM. 2000. Parameter Standar Umum Ekstrak Tumbuhan Obat. Departemen Kesehatan RI, Jakarta.

Esmaeili, N., Ebrahimzadeh, H., Abdi, K., and Safarian, S. Determination of some phenolic compounds in Crocus sativus L. corms and its antioxidant activities study. Pharmacognosy Magazine, 2011, 7(25), 74-80.

Fachriyah, E., Haryanto, I.B., Kusrini, D., Sarjono, P.R., dan Ngadiwiyana. Antioxidant activity of flavonoids from cassava leaves (Manihot esculenta Crantz). Jurnal Kimia Sains dan Aplikasi, 2023, 26(1), 10-18.

Hakim, A. Diversity of secondary metabolites from Genus Artocarpus (Moraceae). Nusantara Bioscience, 2010, 2(3), 146-156.

Herbone, J.B. 1988. Textbook of Phytochemical Methods. A Guide to Modern Techniques of Plant Analysis. 5th Edition, Chapman and Hall Ltd, London; 21-72.

Jayani N, Handojo H. Standarisasi simplisia daun Tempuyung (Sonchi folium) hasil budaya di UBAYA training center trawas mojekerto. Journal of Pharmacy Science and Technology, 2018, 1(1), 68-79.

Kementerian Keseharan Republik Indonesia. 2000. Materia Medika Parameter Standar Umum Ekstrak Tumbuhan Obat. Jakarta. Departemen Kesehatan Republik Indonesia 9-12.

Kementerian Keseharan Republik Indonesia. 2017. Indonesian Herbal Pharmacopoeia. Second Edition. Jakarta.

Lusiana, L. Potensi antioksidasi ekstrak etanol Jamur Tiram Putih (Pleurotus Ostreatus). Gradien: Jurnal Ilmiah MIPA, 2015, 11(1), 1066-1069.

Miller, S. 2005. New Essential Physiology Experimental Design and Statistic Second Edition. London and New York: Routledge.

Murray, P.R.D., Cox, J.H., Chiappini, N.D., Roos, C.B., McLoughlin, E.A., Hejna, B.G., et al. Photochemical and electrochemical applications of proton-coupled electron transfer in organic synthesis. Chemical Reviews, 2022, 122(2), 2017-2291.

Nurulafifah, U. 2017. Uji aktivitas antidiabetes ekstrak etanol 96% buah Pare (Momordica charantia L.) terhadap tikus jantan galur wistar yang diinduksi aloksan. Skripsi. Surakarta: Program Studi Farmasi Universitas Muhammadiyah Surakarta. Jawa Tengah.

Nurhasanah, F. Efek antioksidan dari ekstrak biji Petai Cina (Leucaena leucophala L.) pada tikus putih. Jurnal Ilmu Kefarmasian Indonesia, 2005, 3(1), 13-16.

Padyana, A.K., Gross, S., Jin, L., Cianchetta, G., Narayanaswamy, R., Wang, F., et al. Structure and inhibition mechanism of the catalytic domain of human squalene epoxidase. Nature Communications, 2019, 10(1), 97.

Panche, A.N., Diwan, A.D., and Chandra, S.R. Flavonoids: An overview. Journal of Nutritional Science, 2016, 5, e47.

Rai, S., Kafle, A., Devkota, H.P., and Bhattarai, A. Characterization of saponins from the leaves and stem bark of Jatropha curcas L. for surface-active properties. Heliyon, 2023, 9(5), 15807.

Riasari, H., Sukrasno, and Komar, R. Metabolite profile of various development bread fruit leaves (Artocarpus altilis Parkinson Fosberg) and the identification of their major componens. International Journal of Pharmaceutical Science and Research, 2015, 6(5), 2170-2177.

Riasari, H., Fitriansyah, S.N., and Putra, O. Comparison of anti-inflammatory activity between fermented and dried breadfruit leaves extract (Artocarpus atilis (Parkinson) Fosberg). Pharmacology and Clinical Pharmacy Research, 2018, 3(3), 72-75.

Riasari, H., Febriani, Y., and Nurfauziah, W.A. Toxicity assay of fermented (Artocarpus altilis) leaves using brine shrimp lethality test. Pharmacology and Clinical Pharmacy Research, 2019, 4(2), 46-50.

Riasari, H., Fitriansyah, S.N., dan Hoeriah, I.S. Perbandingan metode fermentasi, ekstraksi dan kepolaran pelarut terhadap kadar total flavonoid dan steroid pada daun Sukun (Artocarpus atilis (Parkinson) Fosberg). Jurnal Sains dan Teknologi Farmasi Indonesia, 2022, 11(1): 1- 17.

Sagatova A.A. Strategies to better target fungal squalene monooxygenase. Journal of Fungi, 2021, 7(1), 49.

Sheel, R., Nisha, K., and Kumar, J. Preliminary phytochemical screening of methanolic extract of Clerodendron infortunatum. Journal of Applied Chemistry, 2014, 7(1), 10-13.

Tay, N.E.S., Lehnherr, D., and Rovis, T. Photons or electrons? A critical comparison of electrochemistry and photoredox catalysis for organic synthesis. Chemical Reviews, 2022, 122(2), 2487-2649.

Vellur, S., Pavadai, P., Pandian, S.R.K., Palanichamy, C., Kabilan, S.J., Sundar, K., et al. Optimization of ultrasound-assisted extraction of bioactive chemicals from Hemidesmus indicus (L.) R.Br. using response surface methodology and adaptive neuro-fuzzy inference system. Food Science and Biotechnology, 2023, 33(2), 327-341.

Wagner, H., and Bladt, S. 1986. Plant Drug Analysis - A Thin Layer Chromatography Atlas. Spring-Verlag, Heidelberg.

Wiraatmaja, I.W. 2017. Mebolisme Pada Tumbuhan. Buku Ajar. Program Studi Agroekoteknologi. Fakultas Pertanian Universitas Udayana. Bali.

Xue, Z., Tan, Z., Huang, A., Zhou, Y., Sun, J., Wang, X., et al. Identification of key amino acid residues determining product specificity of 2,3-oxidosqualene cyclase in Oryza species. New Phytologist, 2018, 218(3), 1076-1088.

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Published

2025-05-31