VALIDASI METODE ANALISIS SENYAWA ETIL p-METOKSISINAMAT DALAM PLASMA DARAH TIKUS PUTIH JANTAN GALUR WISTAR MENGGUNAKAN KROMATOGRAFI CAIR KINERJA TINGGI
Abstract
Ethyl p-methoxycinnamate (EPMC) isolated from the rhizome of kencur (Kaempferia galanga L) is being developed at STFI. Validation of EPMC analysis in blood plasma using High-Performance Liquid Chromatography (HPLC) needs to be conducted to perform in vivo EPMC analysis. This study aims to validate the EPMC analysis method in blood plasma using HPLC. The HPLC column used is column C18, with methanol : phosphoric acid 70:30 and 80:20 mobile phases, a flow rate of 1.0 mL/min, and a maximum wavelength of 310 nm. The blood plasma used was the blood plasma of white male rats of the Wistar strain. EPMC was added to blood plasma and analyzed using HPLC. Parameter validation used is system suitability test, linearity, accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ). The results showed that the optimum mobile phase for analyzing EPMS in plasma was methanol : phosphoric acid with a ratio of 70:30. Based on the validation, the linear regression was 0.998. In the accuracy test, the average value range for the percent recovery is 98% - 102%. In the precision test, the average value of the RSD percentage was 4.10% - 68.83%. The LOD value is 2.747 g/mL, and the LOQ value is 9.158 g/mL. Based on this research, it can be concluded that EPMC in blood plasma can be analyzed using KKKT with column C18 and mobile phase methanol : phosphoric acid 70:30.
References
Fernando, E. 2020. Perbandingan Parameter Bioavailabilitas Etil P- Metoksisinamat (EPMS) Standar Dengan EPMS Isolat Pada Tikus Wistar Jantan. (Skripsi). Sekolah Tinggi Farmasi Indonesia. Halaman 11-12.
Gandjar, I.G., dan Rohman, A. 2007. Kimia Farmasi Analisis.Yogyakarta: Pustaka Utama. Halaman 37-43.
Harahap, Y., Mansur, U., dan Sinandang, T. Analisis Glimepirida Dalam Plasma Tikus. Pharmaceutical Sciences and Research. 2006, 3(1): 22-37.
Harmita. Petunjuk Pelaksanaan Validasi Metode dan Cara Perhitungannya. Majalah Ilmu Kefarmasian. 2004, 1(3): 117-135.
Isadiartuti, D., Prihantini, I.K., dan Agustina, A.P. Development and Validation of an HPLC Method for the Determination of p -methoxycinnamic Acid in Rabbit Plasma. International Conference on Applied Pharmaceutical Sciences. 2018. pp 29-34.
Kurniawati, A. 2016. Validasi Metode Analisis Etil p -Metoksisinamat dalam Plasma secara In Vitro menggunakan Kromatografi Cair Kinerja Tinggi (KCKT). (Skripsi). Fakultas Farmasi. UIN Syarif Hidayattulah Jakarta. Halaman. 4; 26-27.
Mukherjee, A., and Bera, A. A detailed study of validation parameters and system suitability test in HPLC. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2012, 3(4): 426-447.
Rachmaniar, R., Warya, S., Ferdiansyah, R., Riasari, H., and Kenti. Pharmaceutical Cocrystal of Ethyl p-Methoxycinnamate: Formulation and Characterization. 2nd Bakti Tunas Husada-Health Science International Conference. 2020. 6: 96-101.
Setyawan, E., Putratama, P., Ajeng, A.D., dan Rengga, W.D.P. Optimasi Yield Etil p- Metoksisinamat Pada Ekstraksi Oleoresin Kencur (Kaempferia galanga) Menggunakan Pelarut Etanol. Jurnal Bahan Alam Terbarukan. 2012, 1(2): 31-38.
Shargel, L., and Andrew, B.C. 2012. Apllied Biopharmaceutical and Pharmacokinetics (7thed.). The McGraw-Hill Companies. 469-470.
Susanti, M., dan Dachriyanus. 2014. Kromatografi Cair Kinerja Tinggi. Padang: Universitas Andalas. Halaman. 55-67.
Zhang, K., Wenxia, X., and Shuge, T. Comparative Study On The Determination of Ethyl p-Methoxycinnamate In Kaempferia Galanga Rhizome by HPTLCS and HPLC. Journal of Planar Chromatography. 2020, 33(1): 1-7.
Copyright (c) 2022 Revika Rachmaniar, Natalia Suryanata, Nela Simanjuntak
This work is licensed under a Creative Commons Attribution 4.0 International License.
