SINTESIS SENYAWA 4’-HIDROKSI-4-METOKSIKALKON DAN UJI AKTIVITAS ANTIBAKTERI TERHADAP BAKTERI KONTAMINAN PRODUK DARAH
Despite of the blood products are crucial for health, it cannot be separated from the risks of bacterial contamination. The bacterial contamination obtained from collecting and processing blood processing. Various species of positive and negative Gram bacteria are known to be bacterial contaminants in blood products such as thrombocyte concentrate, packed red cell, dan whole blood. Therefore, it is essential to find an antibacterial compound that could inhibited the growth of bacterial contaminant in blood products. Chalcone compounds are known to become one of antibacterial since they have an unsaturated-ketone-group which acts as an antibacterial because it can damage the bacterial cell wall. The presence of hydroxyl group has important role for inhibiting bacterial contaminant growth as well. In this research, 4’-hydroxy-4-methoxychalcone was synthesized through the Claisen-Schmidt condensation reaction. The characterization of the chalcone compounds was carried out by mass spectroscopy using gas chromatography-mass spectroscopy (GC-MS) and identification of functional groups using Fourier transform infrared (FTIR). Bacterial contaminants from blood products that were used: Acinetobacter, Escherichia coli, Bacillus sp, and Staphylococcus aureus. In addition, Staphylococcus aureus from the American Type Culture Collection (ATCC) was used as comparing isolates. The antibacterial activity was analyzed by inhibition area method. The results showed that 4’-hydroxy-4-methoxychalcone successfully synthesized with the reaction product in yellow crystal formation with yield of 72,80%, relative purity 100%, and m/z 254. Antibacterial activity of chalcone compounds at various concentrations of 6.25 ppm; 12.5 ppm and 25 ppm against Gram-positive and Gram-negative bacteria are still relatively weak because the percentage of inhibition ability achieved is below 50%.
Bolarinwa, R.A., Aboderin, O.A., Odetoyin, B.W., and Adegunloye, A.B. Bacterial Contamination of Blood and Blood Components in A Tertiary Hospital Setting in Nigeria. International Journal of Infection Control. 2011, 7(1): 1-6.
Dan, W., an Dai, J. Recent Developments of Chalcones as Potential Antibacterial Agents in Medicinal Chemistry. European Journal of Medicinal Chemistry. 2020, 187: 1-20.
Delaney, M., Wendel, S., Bercovitz, R.S., Cid, J., Cohn, C., Dunbar, N.M., et al. Transfusion Reactions: Prevention, Diagnosis, and Treatment. The Lancet, 2016, 388(10061): 2825-2836
Lestari, E., Matsjeh, S., dan Swasono, R.T. Sintesis Senyawa Turunan Khalkon dan Flavon Berbahan Dasar Vanilin dan Uji Sitotoksik Terhadap Sel Kanker Serviks (Hela), Sel Kanker Kolon (Widr), dan Sel Kanker Payudara (T47D) Secara In Vitro. BIMIPA. 2018, 25(1): 53-65.
Garcia, T.R., de Freitas, T.S., dos Santos, H.S., Bandeira, P.N., Julião, M.S.S., Rocha, J.E., et al. Structural, Vibrational and Electrochemical Analysis and Antibiotic Activity Study of Chalcone (2E)-1-(3ʹ,-methoxy-4ʹ,-hydroxyphenyl)-3-(3-nitrophenyl)prop-2-en-1-one. Journal of Molecular Structure. 2020, 1216: 1-10.
Ikhtiarudin, I., Agistia, N., Frimayanti, N., Harlianti, T., and Jasril, J. Microwave-assisted Synthesis of 1-(4-hydroxyphenyl)-3-(4-methoxyphenyl)prop-2-en-1-one and its Activities as an Antioxidant, Sunscreen, and Antibacterial. Jurnal Kimia Sains dan Aplikasi. 2020, 23(2), 51-60.
Kumar, K.S., Siddaiah, V., Lilakar, J.D., and Ganesh, A. An Efficient Continuous-flow Synthesis and Evaluation of Antimicrobial Activity of Novel 1,2,3-Triazole-Furan Hybrid Chalcone Derivatives. Chemical Data Collections. 2020, 28: 1-13.
Kusumaningrum, S.B.C., dan Sepvianti, W. Identifikasi Bakteri Kontaminan Pada Produk Darah Thrombocyte Concentrate. Syifa Medika. 2020, 10(2): 117-123.
Lentikasari, A.S. 2017. Perbandingan Metode Dilusi Padat dan Cair Pada Uji Sensitivitas Ekstrak Etanol Bawang Putih (Allium sativum) Terhadap Staphylococcus aureus. (Skripsi). Universitas Kristen Duta Wacana.
Magnang, H., Padaro, E., Mawussi, K., Ouenangou, T., Layibo, Y., Kuéviakoé, M.D.I., et al. Quality Control of Red Blood Cell Pockets Produced at Centre National de Transfusion Sanguine de Lomé. Pan African Medical Journal. 2019, 32: 1-8.
Pleil, J. D., & Isaacs, K. K. (2016). High-resolution Mass Spectrometry: Basic Principles for Using Exact Mass and Mass Defect for Discovery Analysis of Organic Molecules in Blood, Breath, Urine and Environmental Media. Journal of Breath Research. 2016, 10(1): 1-10.
Rosa, G.P., Seca, A.M.L., Barreto, M. do C., Silva, A.M.S., and Pinto, D.C.G.A. Chalcones and Flavanones Bearing Hydroxyl and/Or Methoxyl Groups: Synthesis and Biological Assessments. Applied Sciences. 2019, 9(14): 1-17.
Sepvianti, W., dan Kusumaningrum, B.S.C. Synthesis and Antibacterial Activities Test of Chalcone (E)-3-(4- (Dimethylamino)Phenyl)-1-Phenylprop-2-En-1-One Against Bacteria Contaminant Blood Products. Journal of Health. 2021, 8(2): 75-84.
Sepvianti, W., dan Kusumaningrum, S.B.C. Antibacterial Assay of 2’-Hydroxy-4’,6’,4-Trimethoxychalcone and 4-Methoxychalcone Against Gram Positive and Gram Negative Bacteria. Jurnal Riset Kesehatan. 2019, 8(2), 17-20.
Sepvianti, W., Matsjeh, S., dan Purwono, B. 2016. Sintesis dan Uji Sitotoksisitas Turunan Kalkon terhadap Sel Kanker T47D, HeLa dan WiDr Secara In Vitro. (Tesis). Universitas Gadjah Mada. Yogyakarta.
Tjiptoprajitno, N.A., Aryati, A., dan Sudiana, I.K. (2012). Analisis Produk Darah Thrombocyte Concentrate di Palang Merah Indonesia Surabaya. JBP. 2012, 14(3): 145-152.
Zein, A.F.M.Z., dan Sukrisman, L. Proporsi Reaksi Transfusi Akut di Unit Transfusi Rawat Jalan Rumah Sakit Rujukan Tersier di Indonesia. Jurnal Penyakit Dalam Indonesia. 2020, 7(2), 95-99.
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